Fig. 3
From: miR-4324 inhibits ovarian cancer progression by targeting FEN1
MiR-4324 targeting FEN1 and inhibited the expression of FEN1. A A Venn diagram showing the intersection between the predicted downstream genes of miR-4324 by miRDB.org database and the significantly DEGs from GEPIA database. B RT-qPCR detection of expression of LAD1, FEN1, TRNP1, ABRACL and CADM1 in CaOV3 and OVCAR3 cells transfected with Antisense oligo, Bio-miR-4324 mut or Bio-miR-4324 mimic. C RT-qPCR detection of expression of FEN1 in the ovarian cancer tissues and normal tissues. D Correlation analysis between the miR-4324 expression and FEN1 expression in the ovarian tumor tissues. E Measurement of FEN1 expression in ovarian cancer cells lines (SKOV3, CaOV3, and OVCAR3) and normal ovarian epithelial cell (HOSEpiC). F Bioinformatics analysis showed two binding sites sequence of miR-4324 and FEN1 3′-UTR. G Dual luciferase assay was performed in cells co-transfected with plasmids FEN1-WT or FEN1-MUT1 or MUT2 and miR-NC or miR-4324 mimic in CaOV3 and OVCAR3 cells. *, P < 0.05; **, P < 0.001. WT, wild-type; MUT, mutant; NC, negative control