Table 1 Primer sequences for gene expression evaluation using qRT-PCR
Genes | Forward Sequence | Reverse Sequence |
|---|---|---|
CA125 | 5 ‘-ACT GCC ACT GAG CCA ACA AGT TC-3’ | 5 ‘-GAC TGT GCC AAG ACT ATC CGA AGC-3’ |
HE4 | 5 ‘-TCA ACA GAA GGA GGC AAT GTA T-3’ | 5 ‘-CAG CTG CTT AAT CTT ATG CTC G-3’ |
TCF-21 | 5 ‘-CAG CGA TGT GGA GGA CCT TCA AG-3’ | 5 ‘-TCT CCT CGG TGC TCT CGT TGG-3’ |
β-Actin | 5 ‘-CTC CAT CCT GGC CTC GCT GT-3’ | 5 ‘-GCT GTC ACC TTC ACC GTT CC-3’ |
miR-205 | 5 ‘-TCCTTC ATT CCA CCG GAG TCT G-3’ | MRQ 3′ Primer (provided in the kit) |
U6 | 5 ‘-GGA ACG ATA CAG AGA AGA TTA GC-3’ | 5 ‘-TGG AAC GCT TCA CGA ATT TGC G-3’ |