Fig. 1

Effects of different concentrations of Baicalin on cultured KGN and primary granulosa cells in vitro. The KGN and primary granulosa cells were planted in 24-well plates, and conventionally cultured in DMEM/F12 medium supplemented with 10% FBS and 1% antibiotics/antimycotics for 24 h, and then the medium was replaced by DMEM/F12 containing 0.5% FBS and 1% antibiotics/antimycotics for the starvation culture. After 24 h in the starvation culture, different concentrations of Baicalin (0, 10, 20, and 50 μM) were added. The processing start time was set to 0 h, and then observations were made, and photographs taken after 48, 72, and 96 h. The left picture shows the KGN granulosa cell line (A), and the right picture shows the primary granulosa cells (B). Scale bar = 50 μm