Fig. 10
From: Aged mice ovaries harbor stem cells and germ cell nests but fail to form follicles
A. RT-PCR results for stem/germ cell markers. Transcripts specific for pluripotent stem cells (Oct-4, Sox-2, Nanog), germ cells (Mvh) and primordial germ cells (Stella) were detected in RNA extracted from intact 20 months old ovaries B. Detection of early meiotic markers (Mlh1, Scp-1) and marker specific for ring canals (Tex14) is suggestive of ongoing oogenesis. C. Faint expression of Oct-4A detected in RNA extracted from OSE cells but not in whole ovary. Negative control was run to check for genomic DNA contamination