Fig. 1

CircRHOBTB3 is down-regulated in EOC. A Schematic illustration showing the circularization of RHOBTB3 exon 6 and exon 7 forming circRHOBTB3. B Relative expression of circRHOBTB3 in OC cell lines compared with IOSE-80 was measured by qRT-PCR. C The existence of circRHOBTB3 was validated by RT–PCR followed by Sanger sequencing. The green and orange box represents “head-to-tail” splicing sites of circRHOBTB3. D The expression of circRHOBTB3 was detected by real-time PCR in 40 EOCs and 20 normal ovarian tissues. (one-way ANOVA). E. Survival curve depicted the association between circRHOBTB3 with patients’ overall survival time. F/G. Relative circRHOBTB3 and RHOBTB3 mRNA expression were detected after transfection in SKOV3 and OVCAR8 cells by qRT-PCR (Student’s t-test). Quantified values were the mean SD of at least three independent experiments. (*P < 0.05, **P < 0.0, ***P < 0.001, ****P < 0.0001)