Fig. 3

miR-23a-3p is directly targeted by circRHOBTB3. A The binding sites of circRHOBTB3 in miR-23a-3p were depicted. B The relative luciferase activity was assessed by dual-luciferase report assay. (one-way ANOVA). C the relationship between miR-23a and circRHOBTB3 expression in 60 ovarian tissues was analyzed. (Pearson’s correlation analysis). D Fluorescence in situ hybridization (FISH) assay performed in paraffin shows the expression of circRHOBTB3 and miR-23a-3p in EOC. CircRHOBTB3 probe was labeled with FAM, which was the green light. MiR-23a-3p probe was labeled with cy3, which was the red light. The nuclear staining by DAPI was blue under ultraviolet excitation, E Fluorescence in situ hybridization (FISH) assay performed in cell-climbing section shows the co-localization between circRHOBTB3 and miR-23a-3p