Fig. 5

CircRHOBTB3 reversed miR-23a-3p-induced inhibition of PTEN expression in OC cells. A The binding sites of PTEN in miR-23a-3p were depicted. B Luciferase reporter assay showed miR-23a-3p mimics suppressed the activity of PTEN 3’UTR-WT. However, there was no statistical effect on luciferase activity when PTEN 3’UTR-MT and miR-23a-3p mimics were co-transfected. (one-way ANOVA). C, D Relative PTEN expression was detected after transfection by WB (one-way ANOVA). E, F Expression of Akt, p-Akt protein was measured in groups by Western blot. in OC cells. The proportion of Akt/p-Akt was compared according to the relative gray of the bands. (one-way ANOVA)