Fig. 2

HPTLC of SLSO for identification and quantification of β-sitosterol and stigmasterol. A, C Representative images of TLC plate photographed under white light after derivatization with anisaldehyde sulphuric acid reagent. The concentrations of standard β-sitosterol in the image were 1600 and 1800 µg/mL, while for stigmasterol, they were 1000 and 1200 µg/mL. The injection volume of SLSO was 2 µL and the mobile phase used was chloroform:methanol in 9:1 ratio (v/v). B, D Known amount of oil extract of SLSO (Brown line) was compared with known concentrations of standard β-sitosterol (Sky blue lines) and stigmasterol (Dark blue lines). Duplicate loadings of SLSO were done on the same plate. Inset shows the linearity curve of the standard at various concentrations