Fig. 1

Comparison of mutated genes among all sequenced cases. Comparisons of genes with non-synonymous exonic mutations among all cases are shown. Genes in panel (A) include those mutated in samples obtained pre-NACT, post-NACT or at both time points, with only pre-NACT or post-NACT samples included in panels (B) and (C) respectively. Panel (D) includes genes mutated in pre-NACT and post-NACT samples from the same patient (stable within case), while panel (E) includes genes mutated in only post-NACT samples from the same patient (emergent within case). Emergent genes in > 1 resistant cases are highlighted in panel (F). The allele fraction of the detected mutation is shown (reads for variant allele/total reads). * gene mutated in 5/5 cases; **gene mutated in 4/5 cases; ^#gene mutated in > 1 resistant cases but not in sensitive cases; ^&gene mutated in both sensitive cases. Abbreviations: ND No mutation detected, R Resistant case, S Sensitive case