Fig. 3
MAEL induces EMT and up-regulates FGFR4 expression. Western blot analysis showing that the expression of α-catenin, β-catenin and E-cadherin increased while the expression of vimentin and fibronectin decreased in MAEL-silenced ES2 cells, compared to those in corresponding control cells. The expression of α-catenin, β-catenin and E-cadherin decreased while the expression of vimentin and fibronectin increased in stable MAEL-transfected HO8910 cells compared to those in corresponding control cells. B Nine genes (i.e. SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4) were found to exhibit a more than 2-fold different expression in ES2-shMAEL-1 cells compared to that in ES2-shControl cells using a Human Tumor Metastasis RT2 Profiler™ PCR Array. C The expression of SYK, HGF, TIMP3, MMP13, MMP7, FLT4, CXCR4, MMP3 and FGFR4 was verified in ES2-shMAEL-1 cells and their respective controls by western blot. D Overexpression of MAEL and FGFR4 were detected by IHC in a EOC case (200×)