Fig. 2

The effect of FSH on the proliferation and apoptosis of DMSCs. A Different concentrations of FSH acted on DMSCs for 24 h and 48 h by CCK-8 method. It had an inhibitory effect on the proliferation of DMSCs at 100 ng/ml for 24 h (P < 0.05). It had an inhibitory effect on the proliferation of DMSCs at 10, 30, and 100 ng/ml for 48 h (P < 0.05). The results shown are means ± SEM. B Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay to detect apoptotic cell death. Representative microscopic images of TUNEL-positive cells in DMSCs treated with different doses of FSH at 24 h and 48 h. The results showed that high doses and prolonged FSH contributed to the apoptosis of DMSCs. Red = tunnel; blue = DAPI. scale bar = 50 μm (400X magnification)