Fig. 1

CDDP induces changes in mitochondrial fragmentation, apoptosis and oxygen consumption in A2780s cells, but not in A2780cp cells. A2780s and A2780cp cells were cultured with CDDP at (A) different concentrations (0–10 μM, 24 h) or for (B) different duration (0–24 h, 10 μM). Protein contents of Long form (L) and short form (S) of Opa1, Oma1, p-p53 (ser15), Phb1, and GAPDH (loading control) were examined by Western blotting (WB). C and D Morphological assessment of apoptosis was examined by Hoechst nuclear assay in cells cultured above in (C) concentration- and (D) culture duration- dependent manner. E For measurement of mitochondrial respiration, A2780s and A2780cp cells were seeded on 96-well plate, treated with CDDP and resting oxygen consumption rate (OCR) were measured after exposure to biomodulators as indicated (dashed vertical line), using an XF96e Extracellular Flux Analyzer. F OCR measurements were obtained over time (72 min) in A2780s and A2780cp cells cultured in the absence (0 μM) and maximal concentration of CDDP (10 μM). For all experiments described, DMSO was used as vehicle control. Results are expressed as mean ± SEM (n = 3) and analyzed by 2-way ANOVA and Bonferroni post-hoc test. [*p < 0.05, **p < 0.01, ***p < 0.001 (versus absence of CDDP), n = 3]