Fig. 5

CDDP induced mitochondrial p-p53 (ser15) accumulation and binding to Phb1 and Bak in CECA cells. A OV2008 (chemosensitive) and C13* (chemoresistant) cells were treated with CDDP (0–10 μM, 6 h). Mitochondrial, cytoplasmic and nuclear fractions were prepared. Contents of Opa1, Oma1, Phb1, p-p53 (ser15), Bak, Histone H3 (nuclear loading control), Tom20 (mitochondria loading control), and GAPDH (cytoplasm loading control) in the above subcellular fractions were examined by WB. B OV2008 and C13* cells were treated with CDDP (0–10 μM, 6 h). Mitochondria fraction was separated and immunoprecipitated with anti-IgG (control; lanes 1) or anti-Bak antibody. Protein-protein interaction was determined by IP-Western. Bak immunoprecipitates were immunoblotted [IP: anti-Bak, WB: anti-Bak, −Phb1 and -p-p53 (ser15)]. Results are expressed as mean ± SEM (n = 3) and analyzed by 2-way ANOVA and Bonferroni post-hoc test. [***p < 0.001(versus OV2008 treated with DMSO), n = 3]