Fig. 6

CDDP promotes the interaction of Phb1 and p-p53 (Ser 15) in mitochondria in chemosensitive but not chemoresistant OVCA and CECA cells. Examination of mitochondrial interaction of Phb1-p-p53 (ser15) PLA signal in OVCA and CECA cells lines. A2780s and A2780cp OVCA cells A as well as OV2008 and C13 CECA cells (C) were cultured with CDDP (10 μM, 0–24 h). and DMSO is used as a vehicle. Interaction of Phb1 and p-p53(ser15) in the mitochondria and nucleus were assessed by using Duolink Image Tool [Number of PLA unit (A: red spot) with counter staining of DAPI (Blue), and TOM 20 (Green)], (B, D respectively). Results are expressed as mean ± SEM (n = 3) and analyzed by 2-way ANOVA and Bonferroni post-hoc test. [****p < 0.0001 (versus time = 0 h with CDDP); n = 3]