Fig. 7

Chemotherapy-induced mitochondrial interaction of phb and p-p53 (Ser15) in vivo is associated with chemo-responsiveness in OVCA patients. A Images represent PLA signal from pre- and post- chemotherapy in OVCA IHC sections collected from chemosensitive patients (PFI = 77.9 month, mo) and chemoresistant patients (PFI = 2.4 mo), respectively. Scale bar: 10 μm. B & C Paired pre- and post- chemotherapy high-grade serous ovarian tumor sections from the same patients (n = 29, total 58 sections) were stained with anti-Phb1 and anti-p-p53 (ser15) antibodies. B progression free interval (PFI, from the end of 1st chemotherapy to recurrence of cancer), (D) Length of progression free survival (PFS, from starting of 1st treatment to recurrence of cancer),and (C & E) overall survival (OS, from starting of 1st treatment to last follow up) were measured based on its definition during the treatment. Number of Phb1-p-p53 (ser15) interactions (PLA unit: red spot) and its cellular localization [(Blue: DAPI) and (Green: TOM20)] were assessed. Correlation between increased PLA unit [(Phb1-p-p53(ser15)] and PFI (n = 29) (B) and OS (n = 29) (C) was analyzed [Increased mitochondrial PLA unit = Phb1-p-p53(ser15) interaction_{(Post-chemotherapy)} minus Phb1-p-p53(ser15) interaction_{(Pre-chemotherapy)}]. Higher Phb1-p-p53 (ser15) interaction (increased mitochondrial PLA unit) is associated with better PFS (D) but not OS (E), as determined by Kaplan – Meier analysis and hazard ratio (HR) determination. The correlation was analyzed using Pearson (r) method and Kaplan Meier curved were stratified according to the log rank p method