Fig. 3

Tissue distribution of specific immunolabelling for Ca²⁺ release channels InsP₃Rs and RyR in the neonatal ovary (PND 3). A: InsP₃R-specific immunostaining. a, b: Enlarged images from the square areas indicated in A. yellow and red asterisks: strongly InsP₃R-positive oocytes from primordial and primary follicles, respectively. yellow arrows: granulosa cells surrounding primary follicles display labeling at the plasma membrane. blue asterisks: unstained stromal cells. B: RyR-specific immunostaining. c, d: Enlarged images from the square areas indicated in B. yellow asterisks: primordial follicles show moderate cytoplasmic RyR staining. red asterisks: primary follicles. yellow arrows: granulosa cells from primary follicles display moderate labeling near the plasma membrane. blue asterisks: unstained stromal cells. Calibration bar: 100 μm (A, B), 50 μm (a, b, c, d)