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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Expression of voltage-gated Ca2+ channels, Insp3Rs, and RyRs in the immature mouse ovary

Fig. 4

Tissue distribution of specific immunolabelling for CaV1.2 and CaV1.3 voltage-gated Ca2+ channels during the early infantile ovary (PND 8). A: CaV1.2 specific immunostaining. a, b: Enlarged images from the square areas indicated in A. yellow and red asterisks: remaining oocytes from primordial, primary, and secondary, early antral follicles, respectively, are moderately stained. blue arrows: granulosa cells from these follicles show cytoplasmic staining at the plasma membrane and close to the oocyte. yellow arrows: patches of perifollicular cells, possibly immature theca cells are strongly CaV1.2-positive. B: CaV1.3 specific immunostaining. c, d: Enlarged images from the square areas indicated in B. yellow and red asterisks: moderate CaV1.3 immunolabeling of oocytes and granulosa cells from primordial and primary follicles and secondary and early antral follicles, respectively. CaV1.3 positive oocytes and granulosa cells from secondary and early antral follicles. blue arrows: CaV1.3 immunoreactivity of granulosa cells near the plasma membrane. yellow arrows: strongly CaV1.3 positive perifollicular cells surrounding primary, secondary and early antral follicles. Calibration bar: 100 μm (A, B); 50 μm (a, b, c, d)

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