Fig. 6

Tissue distribution of specific immunolabelling for Ca²⁺ release channels InsP₃Rs and RyR in the early infantile ovary (PND 8). A: InsP₃R-specific immunostaining. a, b: Enlarged images from the square areas indicated in A. blue and red asterisks: Oocytes and granulosa cells, respectively, from primary to early antral follicles, show moderate cytoplasmic labeling. yellow arrows: patches of perifollicular cells surrounding secondary and early antral follicles show strong immunostaining. B: RyR-specific immunostaining. c, d: Enlarged images from the square areas indicated in B. The whole ovary expresses RyRs. blue asterisks: oocytes, from primordial to early antral follicles, show punctate cytoplasmic staining. blue arrows: granulosa cells from the same follicles show distinct cytoplasm labeling at the plasma membrane closer to the oocyte. yellow arrows: perifollicular cells are not or weakly RyR-positive. Calibration bar: 100 μm (A, B); 50 μm (a, b, c, d)