Fig. 7
From: Expression of voltage-gated Ca2+ channels, Insp3Rs, and RyRs in the immature mouse ovary
Tissue distribution of specific immunolabelling for CaV1.2 and CaV1.3 voltage-gated Ca2+ channels during the late infantile ovary (PND 16). A: CaV1.2 specific immunostaining. a, b: Enlarged images from the square areas indicated in A. red asterisks: oocytes and granulosa cells from primordial to early antral follicles are moderately stained. yellow arrows: Some oocytes show a strong signal near the plasma membrane. green arrows: granulosa cells, adjacent to the basal lamina are more intensely labeled. blue arrows: patched of perifollicular cells surrounding tertiary and antral follicles show the strongest CaV1.2 staining. B: CaV1.3 specific immunostaining. c, d: Enlarged images from the square areas indicated in B. red asterisks: oocytes, from primordial to pre-antral and antral follicles, show intense cytoplasmic immunostaining at this stage. yellow asterisks: granulosa cells from all follicle stages are CaV1.3 negative. blue arrows: patches of perifollicular stromal cells show moderate CaV1.3 expression. Calibration bar: 100 μm (A, B); 50 μm (a, b, c, d)