Fig. 8
From: Expression of voltage-gated Ca2+ channels, Insp3Rs, and RyRs in the immature mouse ovary
Tissue distribution of specific immunolabelling for CaV2.1 and CaV2.2 voltage-gated Ca2+ channels in the late infantile ovary (PND 16). A: CaV2.1 specific immunostaining. a, b: Enlarged images from the square areas indicated in A. red asterisks: oocytes and granulosa cells from primary, secondary, pre-antral, and antral follicles are express CaV2.1 weakly. blue arrows: in contrast, patches of perifollicular cells forming incomplete envelopes around secondary, early antral, and antral follicles are strongly stained. B: CaV2.2 specific immunostaining. c, d: Enlarged images from the square areas indicated in B. Weak to moderate CaV2.2 labeling is visible throughout the ovary. red asterisks: weakly stained granulosa cells. yellow arrows: most oocytes are weakly stained. green arrows: some oocytes display patches of intense labeling. blue arrows: patches of strongly CaV2.2 positive perifollicular cells form an incomplete envelope around early antral and antral follicles. yellow asterisks strongly CaV2.2 positive bundles of smooth muscle cells seen at the hilum. Calibration bar: 100 μm (A, B); 50 μm (a, b, c, d)