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Fig. 9 | Journal of Ovarian Research

Fig. 9

From: Expression of voltage-gated Ca2+ channels, Insp3Rs, and RyRs in the immature mouse ovary

Fig. 9

Tissue distribution of specific immunolabelling for Ca2+ release channels InsP3Rs and RyRs in the late infantile ovary (PND 16). A: InsP3R-specific immunostaining. a, b: Enlarged images from the square areas indicated in A. yellow asterisks: most oocytes and granulosa cells from primary to antral follicles are weakly stained. red asterisks: the granulosa cells of some follicles show intense “punctate” staining. blue arrows: strongly stained clusters of perifollicular cells surrounding secondary, early antral, and antral follicles. B: RyR-specific immunostaining. c, d: Enlarged images from the square areas indicated in B. yellow asterisks: granulosa cells are weakly stained, regardless of the follicle maturation. blue arrows: oocytes are strongly RyR-positive with aggregates of immunolabelling close to the plasma membrane. yellow arrows: clusters of fat perifollicular cells around early antral and antral follicles show moderate RyR staining. Calibration bar: 100 μm (A, B); 50 μm (a, b, c, d)

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