Fig. 3

IGF1 knockdown represses migration, invasion and EMT of ovarian cancer cells. A The silencing efficiency of shRNAs targeting IGF1 in CAOV4 cells detected by RT-qPCR. B The silencing efficiency of shRNAs targeting IGF1 in CAOV4 cells detected by Western blot analysis. CAOV4 cells were transduced with sh-IGF1. C CAOV4 cell viability detected by CCK-8 assay. D mRNA levels of EMT-related factors CDH1, TJP1, CDH2, FN1, CDH11, MMP2, SNAIL and SLUG in CAOV4 cells determined by RT-qPCR. E Protein levels of EMT-related factors E-cadherin, N-cadherin, Snail and Slug in CAOV4 cells determined by Western blot analysis. F CAOV4 cell migration detected by scratch test. G CAOV4 cell invasion detected by Transwell assay. H Expression of the TGF-β signaling pathway-related proteins TGF-β, P-SMAD2 and SMAD2 in CAOV4 cells detected by Western blot analysis. I Expression of the PI3K-AKT signaling pathway-related proteins P-PI3K, PI3K, P-AKT and AKT in CAOV4 cells detected by Western blot analysis. J Expression of the Wnt signaling pathway-related proteins β-catenin, C-myc and CyclinD in CAOV4 cells detected by Western blot analysis. Data are shown as the mean ± standard deviation of three technical replicates. Data comparisons between two groups were analyzed by independent sample t-test. Data comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. Comparisons of data at different time points were analyzed by two-way ANOVA with Bonferroni post hoc test. ** p < 0.01; *** p < 0.001; **** p < 0.0001