Fig. 4

Effect of WISP1 on downstream signal transduction of IGF1. A Relationship between WISP1 expression and tumor stage of ovarian cancer analyzed by UALCAN website. B Expression of WSIP1 and IGF1 in ovarian cancer tissues analyzed by TCGA database. C Relationship between WISP1 expression and prognosis of patients with ovarian cancer analyzed by UALCAN website. D WISP1 expression in adjacent normal tissues and ovarian cancer tissues determined by RT-qPCR (n = 57; **** p < 0.0001, vs. adjacent normal tissues). E The expression of WISP1 and IGF1 in ovarian cancer tissues (n = 57) was analyzed by Pearson’s correlation analysis. F Silencing efficiency of shRNA targeting IGF1 detected by RT-qPCR. G Silencing efficiency of shRNAs targeting IGF1 detected by Western blot analysis. H The interaction between IGF1 and WISP1 detected by Co-IP. Cells were transduced with sh-WISP1 and/or IGF1. I Expression of EMT-related factors CDH1, TJP1, CDH2, FN1, CDH11, MMP2, SNAIL and SLUG in cells determined by RT-qPCR (*p < 0.05 vs. cells transduced with sh-NC). J Protein levels and phosphorylated levels of IGF1R and IRS1 in cells determined by Western blot analysis. K Protein levels of the Wnt signaling pathway-related proteins β-catenin, C-myc and CyclinD in cells determined by Western blot analysis. L The interaction between αvβ3 and IGF1 detected by Co-IP. Data are shown as the mean ± standard deviation of three technical replicates. Data comparisons between adjacent normal tissues and ovarian cancer tissues were analyzed by paired t-test. Data comparisons between two groups were analyzed by independent sample t-test. Data comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test. Pearson’s correlation analysis was carried out to determine the relationship between indexes. *** p < 0.001; **** p < 0.0001