Table 1 List of antibodies used for Western blot analysis and immunohistochemistry (IHC)
From: Neuregulin-1 signaling regulates cytokines and chemokines expression and secretion in granulosa cell
Peptide/ Protein target | Name of antibody | Name of individual providing the antibody | Species raised (Monoclonal or Polyclonal) | Research Resource Identifier (RRID) | Dilution used |
|---|---|---|---|---|---|
Neuregulin-1β (Nrg1) | Anti-Neuregulin-1β (Nrg1) | Santa Cruz, CA, USA | Rabbit polyclonal | AB_675755 | 1:1000 (WB) 1:200 (IHC) |
ErbB3 receptor | Anti-ErbB3 receptors | Millipore, USA | Mouse monoclonal | AB_309713 | 1:1000 (WB) 1:200 (IHC) |
pErk1/2 | Anti-pErk1/2 | Cell Signaling, Beverly, MA, USA | Mouse monoclonal | AB_2297442 | 1:1000 |
Total Erk1/2 | Anti-Total Erk1/2 | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_331775 | 1:1000 |
pAkt | Anti-pAkt | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_329825 | 1:1000 |
Total Akt | Anti-Total Akt | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_329827 | 1:1000 |
pPI3K | Anti-pPI3K | Cell Signaling, Beverly, MA, USA | Rabbit polyclonal | AB_659940 | 1:1000 |
Total PI3K | Anti-Total PI3K | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_659889 | 1:1000 |
FSH-R | Anti-FSH-R | Abcam, Waltham, MA USA | Sheep polyclonal | AB_2294259 | 1:1000 |
Aromatase (Cyp19A1) | Anti- Aromatase (Cyp19A1) | Abcam, Waltham, MA USA | Rabbit monoclonal | AB_444718 | 1:1000 |
β-Actin | Anti-Beta-Actin | Cell Signaling, Beverly, MA, USA | Rabbit monoclonal | AB_330288 | 1:1000 |