Fig. 5

Circ-BNC2 increases the expression of FBXW7 by targeting miR-223-3p. A TargetScan online tool was used to predict the binding site between miR-223-3p and FBXW7 3′-UTR. B Dual-luciferase reporter gene assay was used to validate the targeting relationship between miR-223-3p and FBXW7 3′-UTR. C RNA pull-down assay was carried out to verify the interaction between miR-223-3p and FBXW7 in SKOV3 and HO-8910 cells. D-E Western blot assay was used to examine the protein expressios of FBXW7 in SKOV3 and HO-8910 cells after transfection. F qRT-PCR was used to detect the expression of FBXW7 mRNA in OC tissues and normal tissues adjacent to tumors. n = 40. G-H Pearson’s correlation coefficient was used to analyze the correlation between miR-223-3p and FBXW7 mRNA, FBXW7 mRNA and circ-BNC2 expression in OC tissues, respectively. **P < 0.01, and ***P < 0.001