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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Hexokinase 2 promoted cell motility and proliferation by activating Akt1/p-Akt1 in human ovarian cancer cells

Fig. 3

HK2 promoted cell proliferation of human ovarian cancer cell lines in vitro. The colony formation assay was used to detected the long-term cell survival and growth in HK2-modified cells and control cells and the quantitative analysis is shown: (A) SKOV3-GFP and SKOV3-HK2; (D) A2780-shCtr and A2780-shHK2. The growth curves were used to detected the cell proliferation in HK2 modified cells: (B) SKOV3-GFP and SKOV3-HK2; (E) A2780-shCtr and A2780-shHK2. The MTT assay were used to detected the cell viability in HK2 modified cells: (C) SKOV3-GFP and SKOV3-HK2; (F) A2780-shCtr and A2780-shHK2. The cell cycle was analyzed in HK2 modified cells by using flow cytometry: (G) SKOV3-GFP and SKOV3-HK2; (I) A2780-shCtr and A2780-shHK2. The mRNA expression of cyclin A1, cyclin D1, cyclin E1, p21, p27 and HK2was detected by real-time quantitative PCR, and the quantitative analysis is shown: (H) SKOV3-GFP and SKOV3-HK2; (J) A2780-shCtr and A2780-shHK2. The protein level of p21 and p27 was detected by western blot and the quantitative analysis is shown: (K) SKOV3-GFP and SKOV3-HK2; (L) A2780-shCtr and A2780-shHK2

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