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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes

Fig. 2

Validation of DEPs by immunofluorescence. a Representative confocal images of fluorescent staining of five candidate DEPs in fresh and aging oocytes, namely MTX2, GDF9, TACC3, EIF5A and ASPM. Scale bar, 20μm. b Intracellular relative fluorescence intensity of MTX2 signal intensity in fresh and aging oocytes. c Intracellular relative fluorescence intensity of GDF9 signal intensity in fresh and aging oocytes. d Intracellular relative fluorescence intensity of TACC3 signal intensity in fresh and aging oocytes. e Intracellular relative fluorescence intensity of EIF5A signal intensity in fresh and aging oocytes. f Intracellular relative fluorescence intensity of ASPM signal intensity in fresh and aging oocytes. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences

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