Fig. 4

3D organoids more closely recapitulate the oxidative stress of in vivo metastases. SKOV3-Hyper-expressing cells were grown as 2D monolayers or 3D organoids and then injected IP and imaged through an intravital window. Representative fluorescence micrographs of cells imaged in (A) 2D and (B) 3D organoids. C An intravital window was positioned against the mouse omentum (indicated by the white arrow), through which (D) omental SKOV3-HyPer cells were imaged in the intravital context. E HyPer fluorescence was quantified. Scale bars = 100 um