Table 1 Biosensors used for this investigation
From: Using genetically encoded fluorescent biosensors to interrogate ovarian cancer metabolism
Metabolic State | Sensor | Excitation/Emission | Sensing Domain | Source |
|---|---|---|---|---|
Intracellular H2O2 | HyPer-DAAO | 420/500 and 480/500 nm | OxyR, H2O2 sensor/transcription factor (Escherichia Coli) | [24] |
NADPH:NADP+ | iNap | 420/528 and 485/528 nm | NAD(H) binding domain of Rex (Thermus aquaticus) | [18] |
ATP:ADP | Perceval | 405/530 and 490/530 nm | ATP-sensing domain from GlnK1 (Methanococcus jannaschii) | [21] |
NADH:NAD+ | Peredox | 405/525 and 587/629 nm | NAD(H) binding domain of Rex (Thermus aquaticus) | [19] |