Fig. 1

The expression of circ_0000144 was increased in ovarian cancer tissues, serum, and cells. The expression of circ_0000144 (A) and SLAMF6 (B) in 60 ovarian cancer tissues and paired adjacent normal tissues. The expression of circ_0000144 (C) and SLAMF6 (D) in 60 patient’s serum or 60 healthy controls was detected by qRT-PCR. Kaplan-Meier analysis showed that OS (E) rate and DFS (F) rate of patients with high circ_0000144 expression. The expression of circ_0000144 (G) and SLAMF6 (H) in cell lines was detected by qRT-PCR. (I) PCR assay with divergent and convergent primers were used for the amplification of cDNA and gDNA samples. In addition, PCR products of circ_0000144 were validated by Sanger sequencing (J). Actinomycin D treatment showed that circ_0000144 was more stable than linear SLAMF6 (K). In addition, RNase R digestion showed that linear mRNA SLAMF6 was digested while circ_0000144 was not (L). *P < 0.05