Fig. 2

GalTKO primary follicle granulosa cells show increased growth and stress with impaired ISR at progressive ages. Dots plotted represent individual follicle granulosa cells measured in each specimen, compared with MW-U; horizontal lines show median values. Frequency distributions are compared with KS. Intensities for (A) P-eIF2ɑ (p = 0.01), (B) BiP (p = 0.0059), and (C) Ki67(p = 0.03) at 5D. Frequency distribution and median values of (D, E) P-eIF2ɑ (p < 0.05, p = 0.06) at 14D. At 14D, fluorescence intensities for (F) Ki67 (p = 0.0008) and representative image of a primary follicle granulosa cell staining (G) in WT and GalTKO ovaries. Circles indicate outer edge of primary follicle granulosa cells with green stars showing the oocyte. Frequency distribution of (H, I) BiP (p = 0.02, p = 0.003) at 30D.