Fig. 3

Anti-cancer effects of PDE10A inhibition in ovarian cancer cells. A Chemical structure for sulindac and its derivative compound MCI-030. B-C Dose-dependent growth inhibitory assays in ovarian cancer cells treated with Pf-2545920 and MCI-030 for 72 h measured by CellTiter-Glo. D Colony formation assays in OV-90 and SKOV3 cells treated with Pf-2545920 and MCI-030 for 12 days. E Cell cycle analysis in TOV112D cells treated with MCI-030 for 24 h using propidium iodide (PI) staining and flow cytometry cell sorting. F-G Time-dependent induction of PARP cleavage, a surrogate marker for apoptotic cell death, was assessed by western-blotting of OV-90 and SKOV3 cells treated with PDE10A inhibitors at increasing time intervals (drug concentration and incubation time as indicated). GAPDH was used as loading control