Fig. 3

Variant p.R349G affected the transcriptional effect of FOXL2. The pcDNA3.1 vector, wild-type (WT) or/and mutant (MT) FOXL2 vectors and CYP17A1 (A)/CYP19A1 (B) promoter reporter were co-transfected in HEK392 cells. The results are shown as luciferase/renilla signal, and the pcDNA3.1 group was used as the control. The potential dominant-negative effect of FOXL2 p.R349G mutation was assessed by co-transfecting WT and MT vectors in 1:1 ratio. * indicated the p-value < 0.05, ** indicated the p-value < 0.01