Fig. 1

Expression of TMEM205 and its involvement in chemoresistance. A SDS PAGE gel stained with Coomassie blue displaying the unique bands in OVTOKO (OV) cells which were sent for protein sequencing. TMEM205 was picked for further evaluation (left panel). Coomassie blue stained SDS PAGE gel for the membrane and nuclear fractions of OVTOKO cells (right panel). B WB for the expression of TMEM205 in eight OCCC human samples. C Real time quantitative PCR based relative mRNA expression of TMEM205 in 10 OCCC tissues. D IHC of tissue from a patient with OCCC showing high expression of TMEM205. E) TMEM205 expression showed in OCCC and normal OSE cell lines. F OVTOKO cells showing the membrane expression of TMEM205 (green, counterstained with DAPI for nucleus and orange cell mask membrane stain) (G) TMEM205 was knocked down in OVTOKO cells. The knockdown was confirmed in two different clones (OV TM Si1 and Si2) using both RT qPCR and WB. We proceeded with OV TM Si1 clone for the further studies. H Cell viability SRB assays were observed with scrambled TMEM205 SiRNA (n = 5, p0.005). I When treated with GFP labeled CP, the OVTOKO cells showed CP localized on the outer membranes of cells (green color) while the OVTOKO TMEM Si cells clearly show CP accumulation in the nuclei (counterstained with red plasma membrane stain)