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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: LncRNA DANCR counteracts premature ovarian insufficiency by regulating the senescence process of granulosa cells through stabilizing the interaction between p53 and hNRNPC

Fig. 3

Knockdown of DANCR inhibits proliferation and promotes aging-related changes in COV434 cells. CCK-8 assays (A) and EdU staining (B) were performed to investigate cell proliferation capability with DANCR knocked down. C, Flow cytometry was performed to detect the cell cycle, indicating that the proportion of G1 phase cells in sh-DANCR group is significantly higher than that of the control group. D, γ-H2AX immunefluorescence staining indicates cells with DNA damage (red fluorescence) and quantify analysis was performed. E, Aging-related proteins p53 and p21 levels between the sh-DANCR and control. *p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: 50 μm

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Journal of Ovarian Research

ISSN: 1757-2215

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