Fig. 3

SAV1 was low expressed in POF and bound to lncRNA-FMR6. A The interaction among lncRNA-FMR6 and SAV1 was detected via RNA pull-down assay. B RIP was performed to evaluate KGN cells extracts with rabbit monoclonal anti-IgG or anti-Ago2. RNA levels in immunoprecipitates were determined by qPCR. **P < 0.01. C Relative levels of SAV mRNA in KGN cells transfected with either FMR6-pcDNA, empty vector, si-NC and si-FMR6, as assessed via RT-qPCR. **P < 0.01. D SAV1 protein levels in KGN cells transfected with either FMR6-pcDNA, empty vector, si-NC and si-FMR6, as assessed via western blotting. **P < 0.01. E Relative levels of SAV1 in follicular fluid of POF patients (N = 24) and controls (N = 24) were assessed via RT-qPCR. **P < 0.01. F Relative levels of SAV1 in OGCs of POF patients (N = 24) and controls (N = 24) were assessed via RT-qPCR. **P < 0.01. G Pearson’s correlation analysis between lncRNA-FMR6 and SAV1 expressions in follicular fluid of POF patients. H Pearson’s correlation analysis between lncRNA-FMR6 and SAV1 expressions in OGCs of POF patients