Fig. 5

mir-379-5p suppresses rat preantral follicular growth. A Inhibition of exosomal release suppressed DHT-induced follicular growth compared to DHT and control on day 2 and 4 of culture. There were no difference in follicular volume between control and GW4869. B, C Transfection of preantral follicle with mir-379-5p mimic significantly suppressed the follicle growth compared to control and DHT. Alexa-647-labelled mir-379-5p mimic was used to confirm successful transfection of granulosa cells in preantral follicle. Preantral follicles (110 to 150 mm diameter) were mechanically isolated from 13- to 14-day old rats and individually cultured in 96 well dish. Exosome release was inhibited with GW4869 treatment (20 mM) for 24 h before DHT treatment (0 to 1 µM, 4 days). Preantral follicles was transfected with mir-379-5p mimic and cultured without or with DHT (1 µM, 4 days), and follicular growth was assessed on day 2 and 4 post-treatment. Results are expressed as change in mean follicular volume ± SEM (n = 15 follicles each). Data were analyzed by two-way ANOVA and tukey post hoc. *P < 0.05; **P < 0.01, ***P < 0.001