Fig. 3

Changes of proliferation and apoptosis in fetal ovary induced by prenatal prednisone exposure (PPE) with different doses or at different time. (A-F) mRNA expression of Ki67, PCNA and Caspase3, n = 12; (G, H) Level of Bcl2/Bax, n = 12; (I) Protein expression of PCNA by IF (the PCNA positive cells stained by green, 400 ×, n = 5). Mean ± S.E.M., ^*P < 0.05, ^**P < 0.01 vs. control. GAPDH, glyceraldehyde phosphate dehydrogenase; PCNA, proliferating cell nuclear antigen; C1, control of PH; PL, prednisone low-dose exposure; PH, prednisone high-dose exposure; C2, control of PE; PE, prednisone early high-dose exposure; C3, control of PT; PT, prednisone terminal high-dose exposure; IF, immunofluorescence