Table 2 Primer sequences used for RT-qPCR
From: Analysis of serum reproductive hormones and ovarian genes in pubertal female goats
Gene name | GenBank accession number | Forward primer | Reverse primer | Product length (bp) |
|---|---|---|---|---|
IGF1 | XM_005680539.3 | GGATGCTCTCCAGTTCGTGT | TGAGAGGCGCACAGTACATC | 161 |
SFRP4 | XM_005679147.3 | TCCTCATCACCCATCCCTCG | GCGCCACTCGTAACACATGA | 111 |
DUSP1 | XM_018065720.1 | CTGCGAACTGTCCCAACCAT | ACACCCTTCCACCAGCATTC | 144 |
RCAN1 | XM_018055891.1 | GCGATCAGGAGAACGAGGAAGAAG | AGTGGATGGGCGTGTACTCTGG | 99 |
PADI6 | XM_018054709.1 | CTTCGACTGCTACCTGACCG | TGTACCAGACGCTGTCAACC | 185 |
β-ACTIN | XM_018039831.1 | CACGGTGCCCATCTACGA | CCTTGATGTCACGGACGATTT | 158 |
GAPDH | XM_005680968.3 | AGGTCGGAGTGAACGGATTC | CCAGCATCACCCCACTTGAT | 259 |