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Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Treatment for ovarian clear cell carcinoma with combined inhibition of WEE1 and ATR

Fig. 6

Effect of AZD1775 and AZD6738 on expression levels of protein related to apoptosis and DNA damage. A & B. Two OCCC cell lines (WT: ES; Mut: KOC7C) were treated with AZD1775, AZD6738, or combination of both and protein expression levels were examined by Western blots. C. Western blot analysis of ARID1A isogenic pair ES2 WTC, ES2 KD after drug treatment. GAPDH was used as loading control. p-CHK1, phosphorylated CHK1; Cas3, caspase 3; p-CDC25C, phosphorylated CDC25C. Bar graphs show quantification of Western blots using ImageJ as described in Methods. (Two-tailed t test: *, p < 0.05, **, p < 0.01, ****, p < 0.0001, compared to control; #, p < 0.05, ##, p < 0.01, ###, p < 0.001, combination compared to single agent; n = 2)

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