Fig. 8

FOXO1 promoted OV cell metastasis in vitro. A Western blotting (left) and PCR (right) analysis of FOXO1 protein and mRNA expression in IOSE and OV cell lines, including OVCAR3, HO8900, A2780, CAOV3, and SKOV3. B Western blotting was performed to evaluate the FOXO1 expression, p62 degradation and, LC3-II accumulation in SKOV3 cells when FOXO1 is knockdown, in response to serum starvation (SS) for 12 h. PCR verification of FOXO1 knockdown was also conducted (left). Western blotting was conducted to determine the FOXO1 expression, p62 degradation and, LC3-II accumulation in CAOV3 cells when FOXO1 is overexpressed, in response to SS. PCR verification of FOXO1 overexpression was also performed (right). C The invasiveness of SKOV3 cells was suppressed by the knockdown of FOXO1, while the invasiveness of CAOV3 cells was promoted by overexpression of FOXO1. Original magnification × 100. D The migration of SKOV3 cells was suppressed by the knockdown of FOXO1, while the migration of CAOV3 cells was promoted by overexpression of FOXO1. The wound-healing process was monitored for 24 h and observed under microscopy. Original magnification × 40. E Measured by the Cell Counting Kit-8 (CCK-8) assay, FOXO1 promoted the proliferation of SKOV3 and CAOV3 cells. The error bar represents the mean ± standard deviation (SD). **p-value < 0.01; ***p-value < 0.001; ****p-value < 0.0001