Fig. 2

Morphological and molecular characterization of EVs. Western blot analysis of EVs marker proteins FLOT1, CD63, and TSG101 and cellular protein contamination indicator CYTc (A). Transmission electron microscope (TEM) imaging shows a clear morphology of EVs with a cross-sectional size of the diameter, scale bar = 200 nm (B). The concentration and median size of the isolated EVs using the NTA analysis (C). The RNA size distribution of EVs shows the presence of the peak of small RNA and the absence of the 18 s and 28 s ribosomal RNA peaks (D)