Fig. 1

Effects of betacellulin (BTC) on Cx43 phosphorylation and expression in SVOG and primary human granulosa-lutein (hGL) cells. (A and C) SVOG (A) or hGL (C) cells were treated with 50 ng/ml BTC for different time durations (5, 15, or 40 min), and the levels of phosphorylated Cx43 protein were examined via Western blot analysis (quantified data were normalized to those of α-tubulin). (A and C) SVOG (A) or hGL (C) cells were treated with 50 ng/ml BTC for different time durations (3, 6, or 12 h), and the protein levels of Cx43 were measured via Western blot analysis (quantified data were normalized to those of α-tubulin). (B and D) SVOG (B) or hGL (D) cells were treated with 50 ng/ml BTC for different time durations (3, 6, or 12 h), and the mRNA levels of Cx43 were examined via RT‒qPCR. Band intensities of western blot staining were measured and quantitatively analyzed using ImageJ 1.42 (NIH, U.S.A.). The experiments were performed in triplicate. Quantification graphs reflect Integrated Density Values of the treatment group divided by the Integrated Density Values of control group. The results are expressed as the mean ± SEM of at least three independent experiments. Values marked with different letters were significantly different (P < 0.05). In detail, if the letters on two columns are different (E.g., “a” vs. “b” or “b” vs. “c”), it means that the difference between the two groups is significant, on the other hand, if the letters on the column of two groups are the same (E.g., “a” vs. “a” or “b” vs. “b”), it means there is no significant difference between two groups. B and BTC, betacellulin; C and Ctrl, control; Cx43, connexin 43