Fig. 4

Effects of kinase inhibitors on BTC-induced increases in PKC activity and phosphorylated Cx43 in hGL cells. (A and B) Primary hGL cells were pretreated with DMSO, 10 µM sotrastaurin (A) or 10 µM AG1478 (B) for 40 min, and the cells were then treated with 50 ng/ml BTC for an additional 15 min. PKC activity was measured with a PKC kinase activity kit. (C and D) Primary hGL cells were pretreated with DMSO, 10 µM sotrastaurin (C) or 10 µM 0126 (D) for 40 min, and the cells were then treated with 50 ng/ml BTC for an additional 15 min. The levels of phosphorylated Cx43 protein were measured via Western blot analysis. The results are expressed as the mean ± SEM of at least three independent experiments. Values marked with different letters were significantly different (P < 0.05)