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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: Betacellulin regulates gap junction intercellular communication by inducing the phosphorylation of connexin 43 in human granulosa-lutein cells

Fig. 5

Localization and distribution of Cx43 in SVOG cells. (A and B) SVOG cells were pretreated with DMSO, 10 µM AG1478 (A) or 10 µM sotrastaurin (B) for 40 min, and the cells were then treated with 50 ng/ml BTC for an additional 15 min. The localization and distribution of Cx43 protein phosphorylation were examined by immunofluorescence microscopy. Cell nuclei were stained with DAPI. The scale bar represents 50 μm. Magnification, 400×. Quantification of the fluorescence intensities were quantified using ImageJ 1.42 (NIH, U.S.A.). The experiments were performed in triplicate. Arrow indicates the position of the phosphorylated Cx43 protein. Fluorescence intensity was calculated as fluorescence intensity of the treatment group divided by the fluorescence intensity of control group. The image background was subtracted by ImageJ software. The results are expressed as the mean ± SEM of at least three independent experiments. Values marked with different letters were significantly different (P < 0.05)

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