Figure 3

Detection on secretion of VEGF from SKOV-3 cells. (A) After co-culture of SKOV-3 cells with chA21 (5 μg/ml), trastuzumab (5 μg/ml) or chA21(5 μg/ml) plus trastuzumab (5 μg/ml) for 12 h, secreted content of VEGF in the medium was detected by ELISA. *, P < 0.01 compared with control. **, P < 0.01 compared with chA21 or trastuzumab alone. (B) VEGF protein expression in the SKOV-3 cells was detected by western blot.