Figure 4

Secretion of LRG1 into conditioned media by ovarian cancer cell line NIH:OVCAR5. A) MS spectrum for LRG1 peptide, ALGHLDSGNR, identified in the spent media of NIH:OVCAR5 cells with 95% (peptide) probability. Conditioned media from the ovarian cancer cell line was concentrated and processed for MSMS analysis. LRG1was identified with low (protein) probability with a single peptide in the complex mixture. The identity of the peptide was confirmed by manual inspection. Peak assignments are indicated. B) m/z for predicted b- and y- ions for peptide ALGHLDSGNR. Highlighted peaks were identified in the spectrum shown in A. C) Western immunoblot of conditioned media from NOSE cell line 1816-575 and ovarian cancer cell line NIH:OVCAR5. 50 µg of concentrated, conditioned media from each cell line was loaded. Position of molecular weight standards, left.