Figure 3From: Hematopoietic-Prostaglandin D2 synthase through PGD2 production is involved in the adult ovarian physiology Prostaglandins synthesis in the ovary upon PGD2 depletion. (A), Levels of PGD2, PGE2 and PGF2α were measured using ELISA in HQL-79 treated or not ovaries (n = 8 for each condition). Results expressed in pg of prostaglandin/ml showed that PGD2 content is significantly decreased (P-value < 0.01) by 65% upon HQL-79 treatment whereas PGE2 and PGF2α contents were not affected; error bars indicate SD of assays done with two dilutions of the eight samples of each group. Expression levels of H-Pgds, Dp2, PPARγ (B) and Cox-1, Cox-2, mPges-2, Ep2, Fp (C) in ovaries of HQL-79 treated (n = 8) or not (n = 8) mice. By real time RT-PCR, no significant difference of Dp2, PPARγ (B) and Cox-1, mPges-2, Ep2, Fp (C) expression level was detectable whereas a large increase of Cox-2 and H-Pgds expression level was measured upon HQL-79 treatment. All the expression level values were normalized to those of Hprt. Data are expressed as means +/- SE (columns and bars); * P < 0.05 vs control.Back to article page