Figure 3From: Investigation of human cationic antimicrobial protein-18 (hCAP-18), lactoferrin and CD163 as potential biomarkers for ovarian cancer Immunohistochemical expression of CD163 in ovarian tissues. Frozen sections were stained by the immunoperoxidase method as described in Materials and Methods. Representative fields were photographed at 200 X magnification. (a) Normal ovarian tissue; (b) benign ovarian tissue; (c) borderline ovarian cancer tissue; (d) grade 1 ovarian tumour; (e) grade 2 ovarian tumour; (f) grade 3 ovarian tumour. Black arrows denote brown CD163 staining in non-epithelial cells. Red arrows indicate unstained epithelial cells. Negative controls from (g) normal and (h) grade 1 tumour. (i) Tissue staining was quantified using Leica QWin Image Analysis Software, determining ratio of stain/whole tissue. Statistically significant results compared with control samples are denoted with * (Mann–Whitney test, P<0.05).Back to article page