RETRACTED ARTICLE: Follicle stimulating hormone modulates ovarian stem cells through alternately spliced receptor variant FSH-R3

Table 1 Primer details and cycling conditions used in the study

	Sequence	Annealing temperature	Amplicon size
	FSH Receptor Transcripts
R1	CATTCACTGCCCACAACTTTCATC	60°C	84 bp
R1	TGAGTGTGTAATTGGAACCATTGGT	60°C	84 bp
R3	TCTCCACTGCTGCACTGTTGGGCT	55°C	382 bp
R3	ATTCAAATACAGGAAATAGAGAAA	55°C	382 bp
	Pluripotent Stem Cell Marker (VSELs)
Oct-4A	CAATTTGCCAAGCTCCTAAA	53°C	290 bp
Oct-4A	TTGCCTCTCACTTGGTTCTC	53°C	290 bp
Sox-2	TGATACGGTAGGAGCTTTGC	56°C	362 bp
Sox-2	CTTTTGCCCCTTTAGAGACC	56°C	362 bp
	Differentiation Marker (OGSCs)
Oct-4 (all isoforms)	GAGCCGAACCCTGAGGAGTCCC	66°C	225 bp
Oct-4 (all isoforms)	CAGCAGGGGCCGCAGCTTAC	66°C	225 bp
	Housekeeping Gene
Gapdh	GCC CAG AAC ATC ATC CCT G	60°C	232 bp
Gapdh	GGT CCT CAG TGT AGC CTA G	60°C	232 bp

Oct-4A is a true marker for pluripotent stem cells [33]. Oct-4 primers amplify both Oct-4A and other isoforms including Oct-4B. Oct-4A reflects VSELs whereas several fold increase in Oct-4 reflects increase in the number of OGSCs suggesting differentiation.

ISSN: 1757-2215